An overview of access provision per facility: CLICK!
1. Structural Audit (no previous experience)
Results of different Structural Audits can be combined in a Structural Audit Report. This could help to enter more challenging and time consuming Enhanced Support and High-End Data Collection modalities.
Mail-in for quality assessment and crystallization – Structural Audit
A macromolecular sample is sent to the biophysics lab of iNEXT partner NKI to be assessed for its general suitability for structural studies. The sample will get checked for quality, quantity, and stability and can be tested for crystallization.
Mail-in SAXS – Structural Audit
A macromolecular sample is sent to iNEXT and a preliminary characterization by SAXS is performed to obtain low-resolution shape characterization and determination of interactions in macromolecular complexes.
Mail-in NMR – Structural Audit
A macromolecular (15N-labeled, Mw < 40 kDa) sample is sent to iNEXT and a preliminary characterization by NMR (15N-HSQC) is performed to obtain information about folding, stability and feasibility for future NMR studies.
Mail-in EM – Structural Audit
A macromolecular sample (Mw > 200 kDa) is sent to iNEXT and negative stain trials for EM studies are performed to obtain information about feasibility for more advanced EM studies.
2. Enhanced Support (non-structural biologists)
From macromolecular sample to X-ray data collection – Enhanced Support
Hands-on support for sample characterization and extensive crystallization trials, leading to structural data via crystallography and/or small angle scattering.
NMR assignment, structure, dynamics – Enhanced Support
Visiting users are supported by local experts in solution or solid state NMR data acquisition, spectral assignments, structure calculations, ligand binding studies, etc. A stable NMR sample is required. Requests can also include combinations of these different NMR activities for a single macromolecule or for related systems or conditions in a single project.
Cryo-EM sample optimisation – Enhanced Support
We will help you to move your project from negative stain images to cryo-EM grids ready for high resolution data collection.
Macromolecular interactions – Enhanced Support
Access to expertise and biophysical equipment to quantify macromolecular interactions. We will help you design, perform and analyse your experiments, and provide 1-2 weeks access to our equipment, including surface plasmon resonance, multi-modal fluorescence plate readers, isothermal titration calorimetry, microscale thermophoresis, stopped flow equipment, multi-angle laser light scattering, and label-free thermal shift assess.
Advanced light imaging – Enhanced Support
Support in the use of advanced light imaging equipment to perform structural studies. For instance for in-cell measurements of protein interaction kinetics.
Ligand and fragment screening – Enhanced Support
iNEXT provides crystallographic screening for validated ligand-binding and fragment-based drug discovery. Access ranges from crystallization trials all the way to data for several crystal structures.
3. High-End Data Collection (expert users)
Following the success of Biostruct-X and its predecessors, this access modality aims to provide synchrotron access to expert users. Note: although “BAG”s are available, all BAG-projects will have to be grouped under a common and coherent research theme.
Following the success of BioNMR and earlier EC-funded NMR initiatives, this modality aims to provide access to expert NMR users. Requests can include the combinations of different NMR activities either for a single macromolecule or for related systems or conditions in a single project.
For the first time, trans-national access to some of the best electron microscopes in Europe for single particle cryo-EM, CLEM, and other experiments is offered.