- iNEXT offers access through three different access themes and eleven modalities through a peer-reviewed process (list of reviewers).
- The submission of iNEXT proposals requires all people involved to have an “iNEXT identity” obtained via Instruct.
By clicking the green ‘Submit Proposal’ button you will be forwarded to the proposal submission system.
By clicking the ‘User Dashboard’ button in the top menu you will be forwarded to your proposals.
- There may be a waiting list for certain popular modalities!
- We recommend that potentially patentable results are not included in proposals until after a patent application has been filed.
- A User Panel was formed, an independent and self-organizing body of well-known structural biologists, to provide feedback to iNEXT. Its Chair that will be a member of the iNEXT Management Committee. Current User Panel members: USER PANEL
1. Structural Audit
Mail-in services for basic sample analysis. The modalities do not include a visit and the analysis is done by local experts.
2. Enhanced Support
The modalities in this theme include structural studies by X-ray crystallography, SAXS or NMR, EM sample optimisation, ligand and fragment screening, advanced light imaging, and the study of macromolecular interactions in vitro or ex vivo (in cells). This type of access is guided by experts from the host institutes and involves training and active participation of visiting users.
3. High-End Data Collection (experts)
Expert users can use some of the best synchrotron beamlines, high frequency NMR magnets and state-of-the-art cryo-EM instruments.
Description of Available Modalities
A macromolecular sample is sent to iNEXT and assessed for its suitability for structural studies. Sample quality, quantity, stability, crystallization potential is evaluated or a preliminary characterization by SAXS is performed. If the sample is suitable for NMR studies (Mw < 40 kDa) or for EM studies (Mw > 200 kDa), the user can request a pre-analysis for NMR, SAXS or EM studies. Results will be combined in a Structural Audit Report that could be of help to enter other modalities.
Cryo-EM sample optimisation
We will help you to move your project from negative stain images to cryo-EM grids ready for high resolution data collection.
Access to expertise and biophysical equipment to quantify macromolecular interactions. We will help you design, perform and analyse your experiments, and provide 1-2 weeks access to our equipment, including surface plasmon resonance, multi-modal fluorescence plate readers, isothermal titration calorimetry, microscale thermophoresis, stopped flow equipment, multi-angle laser light scattering, and label-free thermal shift assess.
Following the success of Biostruct-X and its predecessors, this access modality aims to provide synchrotron access to expert users. Note: although “BAG”s are available, all BAG-projects will have to be grouped under a common and coherent research theme.
Following the success of BioNMR and older EC_funded NMR initiatives, this modality aims to provide access to expert NMR users.
For the first time, trans-national access to some of the best electron microscopes in Europe for single particle cryo-EM, CLEM, and other experiments is offered.
Peer Review and Expected Access Timeline
Proposals can be submitted at any time and are reviewed by a panel of experts that are not associated with the research infrastructures. Proposals are assessed for their scientific merit, but also their value for translational research, their technical feasibility and the quality of the team. The detailed score criteria are:
- Scientific Excellence of the proposed research theme
6. Internationally Outstanding
1. Not Competitive
- Relevance for Translational Research in Health, Biotechnology, or nanomaterials
3. Highly relevant
2. Possibly relevant
1. No translational component
- Scientific Track Record of the team
2. Outstanding team
- Technical Feasibility
1. Not feasible
It is important to notice that a proposal failing any one of the listed criteria (achieving a red score) will be rejected. In addition, it will be necessary to achieve a score of at least 10 to qualify for approval. Some access modalities that are extremely popular will naturally need higher scores to achieve high ranking, while for other cases achieving a qualifying score will be enough. Adjustment to these will be made according to available budgets are perceived popularity by the users.
We aim for rapid turnaround of proposals (less than two weeks). Some of the more complicated proposals (especially in the Enhanced Support access theme) may be pooled and ranked periodically. Following approval, experiments are scheduled by the facilities.